Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑­miRNA‑mRNA ceRNA network by bioinformatics analysis

Long non‑coding RNAs (lncRNAs) are ncRNA transcripts >200 nucleotides that important genetic regulators. LncRNAs can directly regulate mRNA through a lncRNA‑mRNA regulatory mode and also competitive binding to micro (mi)RNA, which is generally known as the endogenous RNA (ceRNA) network. The present study evaluated functional roles networks of lncRNAs in chronic glomerulonephritis (CGN). proliferative ability mouse glomerular mesangial cells (GMCs) induced by different concentrations lipopolysaccharide (LPS) was assessed using Cell Counting Kit‑8 assay, sequencing (RNA‑seq) performed identify differentially expressed LPS‑induced GMCs. Based on results, six were selected for validation reverse transcription‑quantitative PCR (RT‑qPCR). Furthermore, network lncRNA‑miRNA‑mRNA ceRNA constructed assess role mechanism CGN‑related lncRNAs. To elucidate biological functions lncRNAs, Gene Ontology (GO) process term enrichment Kyoto Encyclopedia Genes Genomes (KEGG) pathway analyses all mRNAs involved A total 1,532 including 594 upregulated 938 downregulated identified RNA‑seq. results RT‑qPCR consistent with RNA‑seq results. An network, 236 556 mRNAs, 6 18 miRNAs 419 successfully constructed. GO KEGG demonstrated those often related inflammatory response substance metabolism. key GMCs, further global view CGN. These offered novel insights into pathogenesis CGN potential diagnostic biomarkers.